Figure 1. HIV-1 gag p17/p24 with three epitopes from the HIV ProMix™ peptide pool mapped to its surface.

 

Choose ProMix™, the smarter peptide pool for smart immunology

ProMix™ peptide pools from thinkpeptides consist of a select number of peptides representing the key immunodominant epitopes from a specific protein or pathogen, providing a comprehensive picture of the T cell response. Peptide selection is based on well-established and published epitopes with a wide range of HLA restrictions covering the most relevant HLA types in the population.

 

Unlike peptide pools of protein spanning overlapping peptide libraries, ProMix™ pools allow for a much greater control and purity of its constituent peptide components and avoid overloading cell cultures with large numbers of irrelevant non-responding peptides that can contribute excessive contaminants from peptide synthesis processes.

 

Key advantages of ProMixes™:

• ProMix™ peptide pools use well characterized epitopes to provide a representative picture of the T cell response against a protein or disease.

• Smaller numbers of peptides in each pool are designed to elicit a targeted response based on immunodominant epitopes.

• Uniform >95% purity in pools.

• Peptide pool content is clearly defined, allowing individual peptide responses to be mapped easily if this is required.

• Highly efficient and cost effective method for classifying T cell response.

 

Example Published Data generated with ProMix™ Peptide Pools

Anikeeva N et al.(2016). “Evaluating frequency and quality of pathogen-specific T cells”

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Nature Communications DOI: 10.1038/ncomms13264

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Brief synopsis: Using synthetic peptides and peptide pools Anikeeva et al. have developed a Ca2+ signalling activation assay that offers new insights into the dynamics of antigen-specific T cell activation.